The Hotham River is one of the major tributaries of the Murray River in Western Australia. It is about 160km long in total with its upper reaches being the Hotham River North, which begins in the Dutarning Range and joins the Hotham at its crossing of the Great Southern Highway near Popanyinning. A 15km long southern tributary, Hotham River South begins near Cuballing and flows generally northerly before joining the Hotham near Yornaning.
From Narrogin, the merged river flows in a northerly direction through the Cuballing district and Dryandra Woodland before heading west through Wandering and Boddington.
The river joins the Williams River near Mount Saddleback.
The river was discovered by Thomas Bannister in 1830 and probably named by Governor James Stirling after Admiral Sir Henry Hotham.